Level 4-6

Mammalian Cell Culture Techniques

5 Days

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Level 4-6 Mammalian Cell Culture Techniques - 5 Day Course ( 90 CPD credits)


This course has been approved by the Royal Society of Biology for the purposes of CPD, this course may be counted as 90 CPD points. Click here for more information.


Our Mammalian Cell Culture Techniques course is a rare opportunity to develop hands-on experience with cell culture specialists from pharmaceutical and academic research.

Mammalian Cell Culture is a highly regarded lab technique that is often requested for graduate-level researcher positions in academia and industry. Our Mammalian Cell Culture Techniques course offers comprehensive laboratory-based tuition using a range of human and animal cell lines, thus providing a rare opportunity to pursue further education and future careers in Medicine, Pharmacology, Biology, and Biomedical Science.

This course is designed to introduce students to the fundamental concepts and techniques of mammalian cell culture to help them carry out basic cell-culture techniques properly and safely. The course runs for 5 days of lab-based tuitions and a total of 30 hours of delivery. Working in small groups, students receive comprehensive tuition with experienced scientists covering the laboratory requirements and legal regulations of cell culture in the UK.

Course Content:

  • All aspects of Good Cell Culture Practice (GCCP)- Aseptic technique and contamination control.
  • Sterile media preparation.
  • Passaging of suspension and adherent cell lines.
  • Cryopreservation and resuspension of cell stocks.
  • Analysis of morphology and cell counting.
  • Cell separation and isolation using density centrifugation techniques.
  • Cellular differentiation.
  • Immunofluorescence staining technique.
  • Cell viability assay (commonly used for in vitro drug predictions and nanotoxicology research).

The laboratory results obtained by the students are analysed and discussed. Strong focus is set on the student acquiring a good sterilisation technique through practical training and supervision.

Learning Outcomes:

  • Attendees will benefit from having hands-on laboratory experience in a state-of-the-art industry laboratory at BioGrad Ltd, as well as from interacting closely with our senior scientists and fellow students throughout the course period.
  • Attendees will demonstrate an understanding of the concepts of mammalian cell culture and utilize lab protocols to carry out common mammalian cell culture techniques such as preparing sterile cell culture media, passaging of suspension and adherent cell lines, counting cells using the hemacytometer and the automated cell counter, freezing cells and resuspension of cell stocks,  evaluating confluence and morphology of the cells, and separating of the cells using density centrifugation techniques.
  • Attendees can also measure the cell viability, perform differentiation assay and Immunofluorescence technique as well as hands-on Inverted microscopy.
  • Attendees can design, analyse and present results of a cell-culture-based experiment as well as maintain detailed records of lab work.


  • Students attending this course are expected to have previous laboratory experience, therefore, a questionnaire must be completed prior to the commencement of your course to ensure you are able to meet the required competencies. 

Course Endpoint:

Attendees will gain valuable insights into the range of careers and laboratory research opportunities available. We have links with many companies that recruit interns and graduates based on our recommendations. On completion of the course, your new skills will be endorsed by our lecturers on LinkedIn and a reference can be provided upon request.

All our courses can be booked by paying a deposit and using a payment plan over 1,6 or 12 months if required. For more information please contact our Administration Team via email on: student.services@biograd.co.uk or through the contact form here.

Students attending any of our Level 4-6 and Level 7 courses must be confident and competent in a working laboratory. You must be able to walk in and start work selecting correct pipettes and tips, making molar solutions and serial dilutions to attend these courses. A questionnaire must be completed prior to commencement of your course to ensure you are able to meet these required competencies. If you do not yet possess the necessary skills, we recommend you attend one of our 1 day ‘Laboratory Skills’ courses prior to booking this course.

Our courses are under constant review to ensure they remain inline with current protocols within the NHS, research and industry. This means that course content may change.

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Available Courses

Only 2 places left

Date: August 29, 2022 — September 2, 2022

Location: Liverpool Science Park

Accommodation available?: Yes


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